Bacterial Leaf Streak Quantitative Analysis

The NAGC collaborated with the USDA and university labs to identify Xanthomonas in corn, the causal agent that causes Bacterial Leaf Streak in Corn. As the top corn producing country, there is a strong need to provide US farmers with reliable and early disease detection for a variety of pathogens, particularly for the recently confirmed Bacterial Leaf Streak Disease (BLSD).  BLSD is caused by a bacteria, Xanthomonas vasicola pathovar vasculorum (Xvv), which was thought to be restricted to South Africa. The first reported detection of Xvv in the US was in Nebraska last year (2016) and further surveying has confirmed its presence in several other states (Minnesota, South Dakota, Iowa, Colorado, Illinois, Kansas, Texas and Oklahoma) with more being added (USDA-APHIS, 2016, August 29 and Institute of Agriculture and Natural Resources, University of Nebraska-Lincoln, 2016, August 26). Symptoms of BLSD are similar to other diseases, making visual diagnosis difficult to impossible.

Due to its recent introduction to the US, the epidemiology (control and spread) of BLSD is largely unknown, but it is likely that foliar fungicides typically used against gray leaf spot will be ineffective against this bacterial disease.

Through the USDA’s collaboration, the NAGC has been able to modify the initial assay to allow for a greater number of samples (high throughput) to be analyzed in a shorter period of time. The information gathered will allow for a better understanding of the transmission of the disease and protect producers from further spread within the US. There are only a few research labs that can support and are capable of developing high throughput assays using the latest molecular instrumentation that the NAGC possesses. This assay has the potential to screen other plants that may not yet be identified as alternative hosts (e.g. cotton, food crops or weeds) to the bacteria, which have been important sources in particular strains of Xanthomonas vasicola pathovars (Coutinho, et al., 2015).

Importantly, optimization of the assay will enable producers to test a variety of potential contaminated sources (farming equipment, seeds, soil), which can help market corn for exportation as well as assert liability of contamination from farming equipment that moves from field to field. Furthermore, this assay will provide a more reliable diagnosis, allowing producers to be more proactive in their management strategies.

This project was funded by the National Corn Growers Association and the North Dakota Corn Council.



Green Plant Tissue & Seed Testing

1 – 10 sample submission……………..………………..……..……….$90/sample

For bulk pricing, please contact the laboratory at 701-239-1451.

Soil & Residue Testing

1 – 10 sample submission……………..……………………….……….$120/sample

For bulk pricing, please contact the laboratory at 701-239-1451.


Current Turnaround Time: Less than 30 days

Rush testing is available for a surcharge. Please contact Megan O’Neil at 701-239-1449 for expedited requests.

NAGC Soil Permit 

Submission Form General

Sample Submission -Soil 

Sample Submission – Plant (disease) 

Sample Submission – Seed (disease)