Gray Leaf Spot and Bacterial Leaf Streak Multiplex

Gray Leaf Spot (GLS) is a destructive foliar disease caused by two closely-related fungal species Cercospora zeae-maydis and Cercospora zeina, which have different distributions in North America. C. zeae-maydis is thought to be found throughout North America compared to C. zeina, which appears to be restricted to the eastern Corn Belt. When plants are infected by either species, GLS first manifests as small pinpoints, which eventually turn into brownish-gray, rectangular lesions. Once infected, plants continually shed GLS fungal spores throughout the growing season, which can be transferred to adjacent plants or fields by wind-driven rain. During periods of high humidity and moderate temperatures, large GLS outbreaks can occur. Fungicides have been shown to be effective against GLS. NAGC has developed a real-time PCR multiplex assay for the detection of Cercospora zeae-maydis (Czm) and Cercospora zeina, two groups of pathogens that cause Gray Leaf Spot (GLS) in corn along with the Bacterial Leaf Streak (Xanthomonas) assay. By combining the detection of GLS along with the detection of Xanthomonas vasicola pv. vasculorum, a corn pathogen that causes Bacterial Leaf Streak, into a single assay, allowing for rapid and accurate discrimination of the two pathogens that may be misdiagnosed in corn foliar diseases.

This project was funded by the National Corn Growers Association and the North Dakota Corn Council.

 

 

Pricing

Green Plant Tissue & Seed Testing

1 – 10 sample submission……………..………………..…….……….$90/sample

11 + sample submission…………………………………..…….………$30/sample

Soil & Residue Testing

1 – 10 sample submission……………..…………………..….……….$120/sample

11 + sample submission…………………………..………..…..………$40/sample

 

Current Turnaround Time: Less than 30 days

Rush testing is available for a surcharge. Please contact Megan O’Neil at 701-239-1449 for expedited requests.

 

Submission Form General 

Sample Submission -Soil 

Sample Submission – Seed (disease) 

Sample Submission – Plant (disease)